Protesters swamped the state Capitol after lawmakers sparked outrage last week by re-introducing "the bathroom bill."
That bill would make it illegal for a student to use a bathroom other than the one that corresponds with the gender on their birth certificate.
Those concerned by the bill the most are in the LGBT community.
Their concern is that this would discriminate against transgender students from using the bathroom of the sex they identify with.
Lawmakers last year, pushed the bill off after it drew nation-wide criticism.
Multiple groups canceled their events in Music City in response to talks of the bill last year, and the ACLU even got involved at one point.
The intense scrutiny and controversy over the bill forced it from being voted on, and opponents are hoping for the same thing this year.
Monday's protesters made sure lawmakers knew they were watching by chanting throughout the Senate Chamber.
The controversial piece of legislation was pushed by two Republican lawmakers from Wilson County.
The sponsors, Mark Pody and Mae Beavers, have both remained largely silent since it was filed last week.
When the bathroom bill made its way through committees last year, Governor Haslam expressed concern it would cost the state millions in federal funding.